Deltahelix" pid='20878' dateline='1532400618:
Be more descriptive with your process if you want ppl her to give advice on what you Could/should do differently. Might want to add the amount you plan on making also as that will determine the equiptment to use. As far as sterilizing vials rinse, put unside down on a pan and in the oven @350 celcius for an hour…stoppers in hydrogen peroxide for 10 minutes…
Bro…350 Celsius for an hour? That’s over 650 degrees and sterilization protocol for dry heat sterilizers indicate 375 Fahrenheit for 15 minutes. Sterilizing cycles measured in hours are usually closer to 250 degrees Fahrenheit.
Also, peroxide doesn’t sterilize shit, and setting the vials on the cooking sheet is asinine. Aluminum foil is fully autoclavable and used in sterilizations procedures all the time. Better to set the vials top up with foil over the top. I hope you’re not brewing your own gear.
RickandRoidy" pid='21021' dateline='1532526482:
This guy nailed it. If you’re doing your own gear stick to pre-sterilized vials and syringe filters (assuming you’re doing 10 or less vials at a time). Otherwise you will have to do some shopping around to figure out exactly what your setup should be. The only parts that take any real thought have to do with sterilization and fill levels. Basic starter kit:
- Glass media bottles
- Bottle top filters
- Vacuum pump
- Empty vials, stoppers and caps
- Crimper
- Lab glasswear (beakers etc)
- Autoclave bags
- Carrier oils and solvents
- Pipettes and pipettor
Google, basskiller and Reddit will teach you everything you need to know. Doing this shit is laughably simple, doing it right is only mildly more difficult.