Heating Test C under hot water

[d1444]

What happens if you take Test C oil (or any ester) and run it under steaming/boiling hot water for a very long time?
Would it break down the ester to make the test shorter acting?
Would it destroy the test?

 

[psauce]

Depending on what you mean by "a long time," the answer is not really. Test cypionate was kept at 80 C for 48 hours and no degradation was observed when quantified by HPLC. Elevated temperature may accelerate oxidation kinetics, but in a solution of 5% hydrogen peroxide, no oxidation was observed over several hours.... this is far more oxidizing than oxic headspace within a vial. That means neither the testosterone nor the ester prosthetic group was damaged in either of those cases.

As far as it making a shorter-acting ester, that's now how that would work. The ester linkage, where the cypionate connects to the testosterone, is much less stable than the bonds linking the carbon backbone of the cypionate.

EDIT - Here's the citation. This doesn't mean it's a good idea to store it other than the way people usually do -- cool and dark. You don't know the excipients or impurities in your batch, and this may alter the chemistry in unpredictable ways.

 

[d1444]

As far as it making a shorter-acting ester, that's now how that would work. The ester linkage, where the cypionate connects to the testosterone, is much less stable than the bonds linking the carbon backbone of the cypionate.

Hi,
Can you elaborate more what is meant by this sentence?

Also, does oxidation = degradation?
Simply aweomse reply, thank you.

 

[JamesDoe350]

Depending on what you mean by "a long time," the answer is not really. Test cypionate was kept at 80 C for 48 hours and no degradation was observed when quantified by HPLC. Elevated temperature may accelerate oxidation kinetics, but in a solution of 5% hydrogen peroxide, no oxidation was observed over several hours.... this is far more oxidizing than oxic headspace within a vial. That means neither the testosterone nor the ester prosthetic group was damaged in either of those cases.

As far as it making a shorter-acting ester, that's now how that would work. The ester linkage, where the cypionate connects to the testosterone, is much less stable than the bonds linking the carbon backbone of the cypionate.

EDIT - Here's the citation. This doesn't mean it's a good idea to store it other than the way people usually do -- cool and dark. You don't know the excipients or impurities in your batch, and this may alter the chemistry in unpredictable ways.

please post more medical studies lol thats awesome

 

[psauce]

Hi,
Can you elaborate more what is meant by this sentence?

Also, does oxidation = degradation?
Simply aweomse reply, thank you.

Oxidation is a type of degradation, yeah. A version most people are familiar with is rust of iron.

The other part requires some background chemistry. Testosterone has two bad properties as an injectable drug. It’s not soluble, and the body can use it really quickly if you give it a big amount all at once. To get around both of those, chemists modify the testosterone molecule by attaching a small molecule to the testosterone. That specific linkage is called an ester linkage, and the resulting molecule is a testosterone ester. Test cyp, test enanthate, test prop… those are all testosterone esters, so they’re all testosterone hooked to a different molecule.

The properties of that side chain determine how readily it dissolves as well as how long it takes the body to break the ester linkage — that determines the half-life.

Molecules tend to break in predictable ways, specifically they break at the weakest bonds first. That specific spot where the test is joined to the ester is sort of like a trailer hitch on a truck. The truck is strong, the trailer is strong, and so if shit breaks, you’ll break the hitch before anything. That’s what I meant by saying the carbon backbone is stronger than the ester linkage.

 

[psauce]

I forgot to say why all of this is useful information.

If you don’t like to harpoon yourself with a huge needle, and you don’t want to switch from a loading needle to an injecting needle, you can warm up your test oil to make it less viscous. It goes into the syringe much more easily through small needles if it’s warmed up. Warming up your test will not degrade it, unless there’s something really nasty in the solution that shouldn’t be there.

All of the manufacturers say to avoid light and store at cool temperatures, which is generally good advice, warming it up for injecting won’t have any effect over the lifetime of a vial that you’re using. What you don’t want to do is store a year’s supply in a bright, hot place.